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A simple toll for Raphanus sativus L peroxidase purification and concentration by precipitation with polyacrylate

NadiaWoitovichValetti, Caren Rausch, Guillermo Pic???????³


We present an economical and environmentally-friendly method in which peroxidase fromRaphanus sativus L (wild radish) is separated froma crude extract by precipitation with polyacrylate, a commercially available negatively-charged weak polyelectrolyte. Peroxidase was precipitated by adding polyacrylate at pH 4.00 to the extract. Under these conditions, the enzyme is positively charged and forms non-soluble complexes with the negatively charged polyacrylate. The purification factor of peroxidase was 1.8 with a enzyme recovery of 60%. The volume of the final product was decreased to 10%of the initial feedstock in order to concentrate the sample up to 10 times.


索引于

  • 中国社会科学院
  • 谷歌学术
  • 打开 J 门
  • 中国知网(CNKI)
  • 引用因子
  • 宇宙IF
  • 电子期刊图书馆
  • 研究期刊索引目录 (DRJI)
  • 秘密搜索引擎实验室
  • ICMJE

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