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Analgesic, anti inflammatory and in-vitro cytotoxic effects of andrographis paniculata wall. ex Nees

R.Radha, A.Jerad Suresh, Farheen, B.Uma, M.Lavanya

Objective: To evaluate the analgesic, anti-infllammatory and invitro cytotoxic effects of the ethanolic extract of Andrographis paniculata wall ex Nees. Materials and methods: The analgesic activity of the ethanolic extract of leaves of Andrographis paniculata (EAP) wall.ex Nees were reported to have analgesic activity but the activity has still not reported in stem. The analgesic activity ofAndrographis paniculata was studied in mice by hot plate method, tail clip method and acetic acid induced writhing method. The anti-inflammatory activity of the EAP was studied in rats by carrageenan induced paw edema method. Two different doses of EAP extract (200mg/kg of 400mg/kg bodyweight) of animals were tested. Result: The EAP (400mg/kg) has revealed significant analgesic activity using all the three methods whereas 200mg/ kg extract of AP has shown significant activity only using acetic acid induced writhing method. The EAP extract also revealed significant anti-inflammatory activity. EAP was found to be cytotoxic in the vitro model. The invitro cytotoxicity study showed the IC50 of EAP to be 62µg/ml. Conclusion: The results suggest that the enthanolic extract ofAP possesses analgesic, anti-inflammatory and found to be cytotoxic in the invitro model.