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Antioxidant and antiapoptotic effects of Hydroclathrus clathratus on hepatic dysfunction in alloxan-induced Diabetes mellitus in male albino rats
M.A.Nagy
Background: Hydroclathrus clathratus is considered to have protective effects against several diseases. The hepatic dysfunction associated with Diabetes mellitus (DM) has been reported and was found to be associated with oxidative damage. This study was conducted to evaluate the role of H.clathratus to protect against alloxan -induced liver dysfunction in rats. Method:Alloxanwas administered i.p. in a single dose (150mg/kg) to adult male rats.Alloxan-induced diabetic rats were orally administered hot water extract of H.clathrus (HWHC) 400 mg/kg body weight of rats daily for 30 days after alloxan injection. Result: Alloxan administration to rats resulted in significant elevation of serum transaminases (sALT and sAST), depletion of hepatic reduced glutathione (GSH), catalase (CAT) and glutathione peroxidase (GPx), elevation of lipid peroxides (LPO) expressed as malondialdehyde (MDA). Significant rises in liver tumor necrosis factoralpha (TNF-á) and caspase-3 levels were noticed in alloxan-induced diabetic. Treatment of the alloxan-induced diabetic rats with HWHC significantly prevented the elevations of sALT and sAST, inhibited depletion of hepatic GSH, GPx, CAT and inhibited MDA accumulation. Furthermore, HWHC had normalized serum total proteins and hepatic CAT, TNF- á and caspase-3 levels of alloxan-induced diabetic rats In addition, HWHC prevented the alloxan -induced apoptosis and liver injury as indicated by the liver histopathological analysis. Results showed significant correlation in either alloxan HWHC group between TNF- á and each of serum ALT, AST and liverGPX, CAT,GSH,MDAand caspase-3 levels. Conclusion: our data indicate that HWHC protects against alloxan -induced liver injury in rats through antioxidant, anti-inflammatory and antiapoptoticmechanisms.However, further merit investigations are needed to verify these results and to assess the efficacy of HWHC therapy to counteract the liver dysfunction and oxidative stress status.
