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Characterization of phenolics in two cultivars of broccoli and fennel grown under organic and bio-organic fertilization by high performance liquid chromatography (HPLC)

Alaa A.Gaafar, Zeinab A.Salama, Farouk K.El-Baz

A field experiment was carried out to studythe effect of organic and bioorganic fertilizers on the content of phenolics compound in broccoli and fennel. The resultsindicated the presence of phenolic acids and flavonoids in variable levels. Organic treatments were oriented to increase the accumulation of t phenolic compounds in broccoli and sweet fennel cultivars. Gallic acid, chlorogenic acid, 3,5-dimethoxybenzyl, P-cumaric, quercetin, kampferol and eugenol were found as most abundant consistuents in Calabrese and Southern star cultivars of broccoli. Whereas, Pinostrobin, Pyrogallic acid, Chlorogenic and Protocatechuic acid, were the main phenolic compounds detected in Dolce and Zefafino cultivars treated with organic fertilizer. These results indicated that there is a good margin for enhancing phenolic compounds of broccoli and fennel for economic production using organic fertilization. This study is alsoillustratedthe potential application of broccoli and fennel as a potent natural source of phenolic compoundsas antioxidants andnutraceuticals.