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Determination Of Bergenin In Caesalpinia Digyna By HPTLC

N.Mahadevan, R.Srinivasan, T.Somesh, M.J.N.Chandrasekar, M.J.Nanjan, B.Suresh

A sensitive, rapid, simple and accurate high performance thin layer chromatographic method has been developed to standardize the root of Caesalpinia digyna (Family: Leguminosae) using bergenin as an analytical marker. Precoated HPTLC silica gel GF254 plates were used as stationary phase and ethyl acetate-methanol-glacial acetic acid (8:1.5:0.2, v/v) was used as mobile phase. Rf value of bergenin was 0.22. Detection and quantification were performed by densitometry at 275 nm. The calibration plot was linear in the range of 0.12 μg to 2 μg of bergenin and the correlation coeffecient, 0.999 was indicative of good linear dependence of peak area on concentration. The method permits reliable quantification of bergenin in Caesalpinia digyna. The developed HPTLC method was found to be reproducible, accurate and precise.