抽象的

Development and validation of a highly sensitive spectrophotometric method for the estimation of risperidone in pure and in dosage forms

Hemavathi N.Deepakumari, Hosakere D.Revanasiddappa

The UV spectroscopic method of analysis is widely applicable for routine analytical procedure for the determination of chemical compounds. The present paper describes a simple, highly sensitive and validated UV- spectrophotometric method for the estimation of risperidone (RSP) in both pure and in pharmaceutical preparations. The proposed method is based on the measurement of the absorbance of RSP in THF, which shows maximum absorbance at 285 nm. Calibration graph is linear in the concentration range of 0.5 – 5 ìg/ml with correlation coefficient (r) 0.9994. The apparent molar absorptivity is 6.497 × 104 l/mol/cm. The method gave satisfactory results in terms of repeatability and intermediate precision (RSD< 3.0%). Themethod developed was validated and proved to be robust and rugged. The results showed that this method was successfully applied to the determination of risperidone in tablets, and the results were statistically compared with those of the literature method by employing StudentÂ’s t-test and F- test.The UV spectroscopic method of analysis is widely applicable for routine analytical procedure for the determination of chemical compounds. The present paper describes a simple, highly sensitive and validated UV- spectrophotometric method for the estimation of risperidone (RSP) in both pure and in pharmaceutical preparations. The proposed method is based on the measurement of the absorbance of RSP in THF, which shows maximum absorbance at 285 nm. Calibration graph is linear in the concentration range of 0.5 – 5 ìg/ml with correlation coefficient (r) 0.9994. The apparent molar absorptivity is 6.497 × 104 l/mol/cm. The method gave satisfactory results in terms of repeatability and intermediate precision (RSD< 3.0%). Themethod developed was validated and proved to be robust and rugged. The results showed that this method was successfully applied to the determination of risperidone in tablets, and the results were statistically compared with those of the literature method by employing StudentÂ’s t-test and F- test.