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Evaluation of Phytochemical and Anti-Bacterial Activity of Hot and Cold Methanolic Extract of Leaves and Whole Plant of Andrographis Paniculata

Meenu Sharma, R. G. Sharma, R. D. Rawat and Neetu Sharma

In the present study hot and cold methanol extract of leaves and whole plant of Andrographis Paniculata were screened separately for their anti-bacterial activity against Staphylococcus aureus (MTCC-737) and Escherichia coli (MTCC-452) using Agar well-diffusion method. The susceptibility of the micro organisms to the extracts was compared with each other and with selected standard anti-biotic. It was observed that hot methanol extracts of leaves showed the significant anti-bacterial activity against both bacteria while least bacterial activity was recorded with cold methanolic extracts of whole plant of Andrographis Paniculata. Phytochemical analysis revealed the presence of flavonoids, alkaloids, phenols, glycoside, tannins and saponins.