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High performance thin layer chromatographicmethod for the quantification of lupeol in bark powder of Bauhinia variegata linn

M.B.Kekare, N.Bhandarkar, V.V.Vaidya, G.Charegaonkar


A sensitive, rapid, simple, and accurate high performance thin layer chromatographicmethod has been developed to standardize the bark of Bauhinia variegata Linn.(Family: Caesalpiniaceae) using lupeol as an analytical marker. The ingredients fromthe bark powder were extracted withmethanol which was used for quantification. Chromatography was performed on HPTLC silica gel 60F254 plateswith toluene-acetone-acetonitrile, 9+0.8+0.2 (v/v), as mobile phase. Under these conditions, the Rf of lupeol was 0.58. Quantification was achieved by densitometric scanning at max= 520nm in normalmode. The response to lupeolwas a linear function of concentration over the range 5 to 35g mL-¹. The amount of lupeol in bark powder of B.variegata Linn. was found to be 0.10mg g-¹. The method permits reliable quantification of lupeol and good resolution and separation of lupeol from other constituents of B.variegata Linn. The method was validated for systemsuitability, linearity, precision and accuracy.


索引于

  • 中国社会科学院
  • 谷歌学术
  • 打开 J 门
  • 中国知网(CNKI)
  • 引用因子
  • 宇宙IF
  • 电子期刊图书馆
  • 研究期刊索引目录 (DRJI)
  • 秘密搜索引擎实验室
  • ICMJE

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