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Histopathological Effects of Bifidobacterium spp. in Balbc Mice Infected with Salmonella typhimurium

Naeem AK, Sadiq SM and Sabee KM

The present study involved isolation and characterization of Bifidobacterium from stool of healthy persons and infants. A total of 66 isolates has been collected randomly from healthy persons and infants in Al-Najaf Al-Ashraf city during the period 1/2012-4/2012. Identification of isolates has been carried out depending on their characters on selective media, biochemical tests and polymerase chain reaction technique (PCR). Our results showed that all 66 isolates belong to Bifidobacterium spp., while none of these isolate were belong to B. infantis according to the results of PCR. Furthermore, to explain the role of Bifidobacterium spp. in decreasing infection by pathogenic bacteria, experimental study has been carried out. Salmonella typhimurium were used as pathogens that infect Balb/C mice which were used as a model for experimental study. The result revealed that mice which administered with Bifidobacterium and infected with S. typhimurium showed no important clinical signs of salmonellosis with no pathological of histopathological change when compared with mice that were infected with S. typhimurium (-ve control). Also, the result revealed that Gentamicin had little effect in the treatment of mice administered with Bifidobacterium and infected with S. typhimurium, when compared with mice infected with S. typhimurium only.