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HPLC Analysis and Antioxidant Activities of Holoptelea Integrifolia

Alli Kavitha and Mangamoori Lakshmi Narasu

There has been a renewed interest in naturally-occurring antioxidants from fruits, vegetables and plants. This study is aimed at identifying antioxidant compounds present in the extracts of the Holoptelea integrifolia. The antioxidant properties of methanol, acetone and ethyl acetate extracts of Holoptelea integrifolia were determined using the 2,2-DPPH method, FRAP method and reducing power assay. The total phenolic content was estimated through the use of the folin-ciocalteu method. The total flavonoid content was determined according to Kim et al.1 method. Ascorbic acid, Gallic acid and quercetin were used as standards for evaluating radical scavenging activity, total phenolic content (TPC) and total flavonoid content (TFC). Results derived from the 2,2-DPPH method, FRAP method and reducing power assay showed that the stem extracts of acetone had the highest antioxidant activity. The TPC and the TFC values also showed similar results. The HPLC analysis of the extracts also confirmed the trend derived from the assays. The HPLC analysis showed large quantities of gallic acid and rutin in plant extracts. Results showed that the stem extract of acetone and the leaf extracts from methanol had the highest antioxidant activity compared to other extracts. The present study is the first report on antioxidant properties and phytochemical analysis of Holoptelea integrifolia.