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Hydrolytic degradation monitoring of voriconazole by derivative spectrophotometric method

Mrinalini C.Damle, Amol B.Khetre, Rahul S.Darekar, Shradhanjali M.Singh

A derivative spectrophotometric method for the simultaneous determination of Voriconazole and its hydrolytic degradation product has been developed. The objective of this study was to evaluate the utility of derivative spectrophotometry for the determination of Voriconazole Working standard (VORIWS) in presence of its degradation product without prior separation. First derivative spectrophotometry allowed specific determination of VORIWS at 269.8nm and 234.2nmwith no contribution by the products formed after alkali and acid treatment respectively. Similarly, both acid and alkali catalyzed hydrolytic products were determined at 325nm with no interference byVORIWS. The BeerÂ’s law is obeyed in the concentration range of 50-150mgmL-1 for both VORIWS and its hydrolytic product. The percentage recovery forVORIWSwas found to be 99.16-101.23%. LOD and LOQ values were also determined. Intra and interday precision values were within range. The results indicate that proposed method is simple, rapid and specific.