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Isolation and characterization of flavonoids and ascorbic acid from aqueous extract of leaves of Ocimum sanctum L.

M.S.Kondawar, S.B.Bhise


Ocimum sanctum Linn., a holy plant in India, has given out a special status because of its various pharmacological activity. It has nearlymore than 250 activities reported in different articles. Its leaf contains several chemical constituents of which flavonoids and ascorbic acid are the most important constituents. Due to antioxidant activity of these chemicals they are more popular in maintaining health and protection. But as the constituents can be changed as per the environment, the study was carried out on the leaves of Ocimum sanctum L. for the presence of flavonoids and ascorbic acid. From HPTLC fingerprint analysis it was found to contain four major flavonoids in a fixed ratio as well as it was found to be a good source of ascorbic acid.


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