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Liquid chromatography-tandem mass spectrometric assay for determination of losartan in human plasma

G.Rajender, N.G.B.Narayanan

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has proved to be a powerful research tool due to its sensitivity, high selectivity, and high throughput efficiency. Determination of Losartan in human plasma method was developed and validated.Losartan was extracted from plasma by two-step extraction procedure using chloroform as extracting solvent. In this study, ESI- sense was chosen as the ionization source. Signal intensity was high using ESI- source provided for the quantification of samples. Chromatographic separation was performed on phenomenax C- 18 column (250ï‚´4.60mm5microns).Mobile phase contains acetonitrile, water (70; 30 v/v) + 0.1%acetic acid, flowrate 0.8mL/min.The retention time of Losartan 5.1 min, the total run time 6 min.Linearity correlation coefficients (r2) curve was 0.999818,calibraction range 10-1000ng/mL.TheLLOQ of Losartan 10 Pico gram. The UV detection of Losartan was at 225 nm.MRM (Multiple reactionmonitoring) transition of Losartanm/z 421.28-126.99 was selected to obtain maximum sensitivity. LC-MS/MS method has been successfully used in the pharmacokinetic analysis of Losartan in human serum.