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Multienzymatic Cascade Reactions Via Immobilization Of Enzyme Complexes

Reny Yuong

Multienzymatic cascade reactions are a crucial technology for industrial process development, such as pharmaceutical, cosmetic, and nutritional component synthesis. Various multienzyme structure construction methodologies have been widely described. Fusion proteins, enzyme scaffolds, and immobilisation are three methods for creating enzyme complexes. As a result of substrate channelling, enzyme complexes can boost cascade enzymatic activity. Recent breakthroughs in materials science have led to the creation of a variety of materials that can be used to immobilise enzymes. This review looks at how random coimmobilization, compartmentalization, and positional coimmobilization can be used to assemble multienzyme complexes.