抽象的

Prevalence and identification of toxic shock syndrome toxin producing wound isolates of staphylococcus aureus from Namakkal District of Tamil Nadu

V.Zambare, M.Ponraj, V.Rubeni

Nine- skin infection, eight- burn and nine- accident wound samples were analyzed for the isolation of Staphylococcus aureus on selective Mannitol salt agar (MSA) media and identified by biochemical tests. Out off 31 samples, 10 samples showed presence of S. aureus and out of which burn samples had highest (50%) prevalence of S. aureus. All S. aureus showed multiple antibiotic resistance especially against penicillin (100%), vancomycin (90%),methicillin (80%) and oxacillin (60%). The lowest resistance (30%) was against chloramphenical, erythromycin and trimethoprim. Highest antibiotic resistance of 74, 56.6 and 33.3%were obtained fromaccident wound samples, burn samples and skin infection isolates, respectively. Among the 10 isolates, 5 isolates produced the toxic shock syndrome toxin (TSST) gene and was confirmed by PCR amplification. TSST gene was present only in skin infection samples and few burn samples but not in accidental wound samples.All 5 isolateswith TSST gene were also producing toxin TSST and was confirmed by SDS-PAGE. To the best of our knowledge this is the first report on PCR detection of the TSST-1 gene in S. aureus fromNamakkalDistrict of TamilNadu, India.