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Quantitation of -sitosterol fromWoodfordia fruticosa (Linn.)Kurz and a polyherbal formulation used for treating female reproductive disorders

Smruti Shah, Sunita Shailajan

Woodfordia fruticosa (Linn.)Kurz (syn.W.floribunda Salisb.); Family-Lythraceae, commonly known asDhataki, is a woody shrub distributed throughout India. The flowers are commonly used in the management of female reproductive disorders. A sensitive, simple, and accurate high-performance thin-layer chromatographic method has been established for quantitation of -sitosterol in plant powder of Woodfordia fruticosa (Linn.) Kurz (flowers) and in Wama tablets, a polyherbal formulation used against female reproductive disorder. The plant powder was extracted with methanol and used for quantitation. The concentration of -sitosterol was found to be 0.18g mg-1 in the plant powder ofWoodfordia fruticosa (flowers) and 0.15gmg-1 in the polyherbal formulation. Quantitationwas carried out on HPTLC silica gel 60 F254 pre-coated plates with the mobile phase toluene-ethyl acetate- methanol-glacial acetic acid, 8+1+0.5+0.3 (v/vv/v).ATLC scanner set at 366nmin fluorescence/reflectancemode was used for quantitation. -sitosterol response was linear over the range 10g mL-1 to 70g mL-1. The method was validated for linearity, precision, accuracy and robustness.