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Separation and Characterization of Anthraquinone Derivatives from Cassia Fistula using Chromatographic and Spectral Techniques

Jignasu P. Mehta


Present study involves characteristic evaluation of medicinally important ingredients such as anthraquinone derivatives from Cassia Family. Cassia family is well-known source of anthraquinone glycosides and its derivatives in the various parts of plants. A simple high performance liquid chromatography (HPLC) method was developed and validated for the determination of three anthraquinone derivatives (rhein, emodin and chrysophanic acid) in the extracts from Cassia fistula. The extracts were analyzed on C-18 column isocratic mobile phase in HPLC equipped with UV detector at 254 nm. The identification of each analyte was performed by use of standards. The limits of detection obtained for the analyte were in the range of 2.5-15 μg/mL. All three components present in the extracts of cassia fistula were also characterized by the 1H-NMR and mass spectroscopic analysis


索引于

  • 中国社会科学院
  • 谷歌学术
  • 打开 J 门
  • 中国知网(CNKI)
  • 引用因子
  • 宇宙IF
  • 米亚尔
  • 秘密搜索引擎实验室
  • 欧洲酒吧
  • 巴塞罗那大学
  • ICMJE

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