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Simple and selective spectrophotometricmethods for the determination of hydralazine hydrochloride in bulk drug and in pharmaceutical formulations based on the oxidation-reduction reactions

K.Siddappa, Mallikarjun Metre, Tukaram Reddy, Mahesh Tambe

Two simple, sensitive, accurate and cost effective spectrophotometricmethods have been developed for the determination of Hydralazine Hydrochloride (HLH) in bulk drug and in pharmaceutical formulations. These two methods are based on their oxidation of 1-10 Phenanthroline (Method A) and 2, 2'- Bipyridyl (Method B). The colored complexes formed were measured at 510 and 523 nm respectively against the reagent blank. Reaction conditions have been optimized to obtain colored complexes of higher sensitivity. The absorbance was found to increase linearly with increase in concentration of HLH. The complexes obeyed BeerÂ’s Law in the concentration range of 0.1-1.0 g/ml and 0.1-1.5 g/ml for both the methods. The molar absorptivity, SandellÂ’s sensitivity, detection limit, limit of quantification and correlation coefficient (r) of themethodswere found to be 1.723ï‚´104, 0.1141g/ml0.085µg/ml, 0.2830 g/mland0.9996formethodAand 1.253ï‚´104, 0.01569 g/ml, 0.082 µg/ml, 0.2730 µg/ml and 0.9994 for method B respectively. The optimum reaction conditions and other analytical conditions were evaluated. The common excipients and additives did not interfere with their determinations. The results obtained by the proposed methods were further ascertained by parallel determination by a reference method.