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Simultaneous RP - HPLC Method Development and Validation of Levofloxacin and Ornidazole in Combined Pharmaceutical Dosage Forms

Ch. Narasimha Raju Bh, K. V. Ramana, G. Devala Rao and Parthasarathi Ramamoorthy Thoddi


A simple, precise RP–HPLC method was developed for the estimation of levofloxacin and ornidazole in combined tablet formulation. The quantification was carried out using a Phenomenex C18 column 250 x 4.6 mm i.d, 5 μm particle size in isocratic mode, with mobile phase comprising of 0.1% v/v phosphate buffer pH 3.0 ± 0.05, acetonitrile, methanol in the ratio of 70 : 10 : 20 (v/v/v). The flow rate was 1 mL/min and the detection was carried out by UV detector at 295 nm. The retention times were 3.45 min and 6.67 min for levofloxacin and ornidazole, respectively. The method produced linear response in the concentration range of 40-60 μg/mL and 80-100 µg/mL for levofloxacin and ornidazole. The percentage recovery was found to be 98.79% and 99.47% for levofloxacin and ornidazole, respectively. The developed method was found to be accurate, precise, sensitive and rapid for the simultaneous estimation of levofloxacin and ornidazole in tablets.


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