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Simultaneous spectrophotometric estimation of norethindrone acetate and ethinyl estradiol in formulation

KoneruAjitha, RituKimbahune, .G.Mubeen,Yajnesh Pai


A combination of second order derivative spectrophotometric method and absorbance correction method has been proposed for simultaneous spectrophotometric estimation of Norethindrone Acetate and Ethinyl Estradiol. Amplitude of second order derivative at 266.8 nm was used for quantitation of NorethindroneAcetate and Ethinyl Estradiol was estimated by absorbance correction method after correcting the absorbance for Norethindrone Acetate at 280 nm. The content of Ethinyl Estradiol and Norethindrone Acetate were found about 105.77% and 101.56% in tablet mixture respectively. The method has been validated in accordance with ICH guidelines and the results for all parameters were found to be within the acceptance criteria.


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