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Spectrofluorimetric And Spectrophotometric Determination Of Cephradine And Cefaclor

Suzy M.Sabry, S.F.Belal, Essam F.Khamis, Dina A.Gawad

Simple, accurate, precise and sensitive spectrofluorimetric and spectrophotometric methods were developed for determination of α-amino acyl β-lactam cephalosporines; cephradine(CPHD) and cefaclor(CFL). Both methods are based on coupling reaction of the cited drugs with 4- chloro-7-nitro-2,1,3-benzoxadiazole(NBD-Cl) in 0.1 M sodium borate buffer of pH 8-8.5 to yield yellow fluorescent products. The fluorescence was measured in methanolic-aqueous medium (λex/em=458/528 and 490/538 nm for CPHD and CFL, respectively). The absorbance of the colored products was measured in aqueous solution at 450-455nm. All variables affecting the color development and the fluorescence emission were investigated and conditions were optimized. The calibration plots of CPHD and CFL were linear over the concentration ranges of 0.025-0.3μg ml-1 for the spectrofluorimetric method and of 10-60 and 5-30μg ml-1, respectively for spectrophotometric method. The proposed methods were applied for the determination of CPHD and CFL in bulk and commercial dosage forms and the results obtained were compared statistically with those given by reference methods.