抽象的

Stability and activity behavior of human serum albumin doped in silica sol-gel thin film

Nizam M.El-Ashgar, Zeyad J.Yasseen, Mohammed Y.Abu-Kmail


Transparent sol-gel thin films immobilizedwith human serumalbumin (HSA) were made via the acid catalyzed sol-gel reaction of tetraethylorthosilicate in presence of the human serum albumin. Different surfactants include; cationic cetyl trimethyl ammoniumbromide (CTAB), anionic sodiumdodecyl sulfate (SDS) and nonionic Triton X-100 (TX-100) were tested for the improvement of the host material mesostructure, increasing its porosity and well accommodation of the HSA protein within the silica matrix. The thin films show similar behavior in presence of surfactants as their free counterparts in aqueous solution with a significant shift in the wavelength of absorption. The activity behavior of the immobilized human serum albumin retained its activity by immobilization.Different parameters include concentration of protein and surfactant, type of surfactant, life time and number of measurements were investigated. The HSA thin film sensor showed stability, repeatability, reproducibility and long life time behavior. Maximumstability of HSA thin films were achieved by drying at 37 oC.


索引于

  • 中国社会科学院
  • 谷歌学术
  • 打开 J 门
  • 中国知网(CNKI)
  • 引用因子
  • 宇宙IF
  • 研究期刊索引目录 (DRJI)
  • 秘密搜索引擎实验室
  • 欧洲酒吧
  • ICMJE

查看更多

期刊国际标准号

期刊 h 指数

Flyer