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Study on Interaction of cefoperazone sodium with ofloxacin in the presence of BSA by fluorescence spectroscopy

Baosheng Liu, Chunli Xue, Jing Wang, Chao Yang, Yunkai Lv

Interactionmechanismof cefoperazone sodium(CFP) and ofloxacin (OFX) with bovine serumalbumin (BSA) at different temperatures by fluorescence spectrometry method was studied. Results showed that CFP or OFX could quench the fluorescence of BSA. The fluorescence would quench to a larger degree when OFX (or CFP) was added to the systemof BSA-CFP (or BSAOFX). The quenchingmechanismof the combination for BSAand drugswas a static procedure. The number of binding sites is 1 in various systems. The values of nH were approximately equal to 1. Itwas proved that the interaction betweenCFP andOFXsimultaneously bound to BSAwasweaker and almost no cooperativeness occurred. The sitemarkers competitive experiments indicated that the binding of CFP and OFX to BSA primarily took place in site I. Thermodynamic parameterswere also used to identify the force type of drugs withBSA.